Principal Investigator Peter So
A variety of intracellular transport and trafficking processes, such as endocytosis, exocytosis, and bacterial/viral invasion, are of fundamental biological importance. We are developing two new methods to quantify these processes. The first technique is two-photon single particle tracking. This technique allows the trajectory of a single particle to be monitored with nanometer spatial resolution and millisecond time resolution inside living cells. The second technique is two-photon fluorescence correlation spectroscopy that allows the quantification of the number density, the diffusion rate, and the flow rate of particles being transported inside cells.