Principal Investigator Forest White
To interrogate the signaling networks in these diverse biological systems, we use hybrid quadrupole time-of-flight mass spectrometers coupled with stable-isotope labeling, affinity chromatography, and LC-MS/MS to quantify temporal dynamics of tyrosine phosphorylation on hundreds of proteins simultaneously with site-specific resolution typically from several million cells. After gathering and analyzing the data, we are working with the Lauffenburger and Tidor labs in the Biological Engineering Division at MIT to develop better methods of computational analysis and modeling of signaling networks. These models will then be used to predict biological and signaling network responses to additional perturbations to the system.